Regulatory
Part:BBa_K2314212:Design
Designed by: Lei Zhu Group: iGEM17_OUC-China (2017-10-22)
UTRrpsT enhanced promoter
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 56
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Any modifications involving the 5'UTR need to be considered whether or not a special mRNA secondary structure will be produced. The preliminary analysis of the secondary structure can be carried out by structural prediction software.The RBS sequence is TTGGGATGG.
Source
The researcher claimed that the PUTRs were precisely amplified without superfluous sequences based on the E. coli K12 transcriptional regulatory database, RegulonDB39。In addition the promoter comes from the kit plate。 Reference:【Zhou, S., Ding, R., Chen, J., Du, G., Li, H. Z., & Zhou, J. (2017). Obtaining a panel of cascade promoter-5'-utr complexes in escherichia coli. Acs Synthetic Biology, 6(6).】